足球彩票

您好,欢迎进入上海纪宁实业有限公司网站!
一键分享网站到:
您现在的位置:足球彩票 >> 技术文章 >> 丰寿生物:组织/细胞RNA快速提取
丰寿生物:组织/细胞RNA快速提取
浏览次数:251发布日期:2019-08-23

足球彩票FENGSHOUSHENGWU:ZUZHI/XIBAORNAKUAISUTIQU  CAOZUOBUZHOU:(SHIYANQIANQINGXIANYUEDUZHUYISHIXIANG)

TISHI:

SHIYONGQIANQINGXIANZAIPIAOXIYERWPINGHE70%YICHUNPINGZHONGJIARUZHIDINGLIANGWUSHUIYICHUN!

足球彩票CAOZUOQIANZAILIEJIEYERLTZHONGJIARUβ-QIUJIYICHUNZHIZHONGNONGDU1%,RU1 ml RLT ZHONGJIARU10μl β-QIUJIYICHUN。CILIEJIEYEXIANYONGXIANPEI。PEIHAODERLT 4℃KEFANGZHIYIGEYUE。

1. ZUZHIPEIYANGXIBAO

足球彩票a. SHOUJI<107XUANFUXIBAODAOYIGE1.5mlLIXINGUAN,DUIYUTIEBIXIBAO,KONGBANPEIYANGKEYIZHIJIELIEJIE,XIBAOPINGPEIYANGYINGGAIXIANYONGYIDANBAIMEIXIAOHUAHOUCHUIDAXIALAISHOUJI。

b. 13,000rpmLIXIN10MIAO(HUOZHE300gLIXIN5FENZHONG),SHIXIBAOCHENDIANXIALAI。WANQUANXIQISHANGQING,LIUXIAXIBAOTUAN,ZHUYIBUWANQUANQISHANGQINGHUIXISHILIEJIEYEDAOZHICHANLIANGCHUNDUJIANGDI。

足球彩票c. QINGDANGUANBIJIANGXIBAOCHENDIANWANQUANSONGSANZHONGXUAN,JIARU350μl(<5x106XIBAO)HUOZHE600μl(5x106-1x107XIBAO)LIEJIEYERLT,CHUIDAHUNYUNHOUYONGSHOUJULIEZHENDANG20MIAO,CHONGFENLIEJIE。

d. YONGDAIDUNZHENTOUDEYICIXING 1 ml(PEI0.9mmZHENTOU) ZHUSHEQICHOUDALIEJIEWU 5-10 CIHUOZHIDAODEDAOMANYIYUNJIANGJIEGUO(HUOZHEDIANDONGYUNJIANG30MIAO),KEYIJIANQIEDNA,JIANGDIZHANCHOUDUHETIGAOCHANLIANG。

e. JIECAOZUOBUZHOUXIANGXIA3。

2. DONGWUZUZHI(LIRUSHUGANNAO)

a. DIANDONGYUNJIANG:XINXIANZUZHIYONGJIEPOUDAOXUNSUQIECHENGXIAOSUIKUAI,JIARU350μl(<20mgZUZHI)HUOZHE600μl(20-30mgZUZHI)DELIEJIEYERLTHOUDIANDONGCHEDIYUNJIANG20-40MIAO。

b. YEDANYANMO+YUNJIANG:ZAIYEDANZHONGYANMOZUZHICHENGXIFENHOU,QUSHILIANGZUZHIXIFEN(20mg/30mg)ZHUANRUZHUANGYOU350μl/600μlZUZHILIEJIEYERLTDE1.5mlLIXINGUANZHONG, YONGSHOUJULIEZHENDANG20MIAO,CHONGFENLIEJIE。YONGDAIDUNZHENTOUDEYICIXING 1 ml(PEI0.9mmZHENTOU) ZHUSHEQICHOUDALIEJIEWU 10 CIHUOZHIDAODEDAOMANYIYUNJIANGJIEGUO(HUOZHEDIANDONGYUNJIANG30MIAO),KEYIJIANQIEDNA,JIANGDIZHANCHOUDUHETIGAOCHANLIANG。

c. JIANGYUNJIANGHOULIEJIEWU13,000rpmLIXIN3FENZHONG,CHENDIANKENENGCUNZAIDELIEJIEKUNNANDESUIPIANHUOZHEBURONGWU, JIANGLIEJIEWUSHANGQINGXIAOXINZHUANDAOYIGEXINLIXINGUAN。

足球彩票d. JIECAOZUOBUZHOUXIANGXIA3。

足球彩票3. JIAOGUJILIEJIEWU(SHANGQING)TIJI,JIARUDENGTIJIDE70%YICHUN(QINGXIANJIANCHASHIFOUYIJIARUWUSHUIYICHUN!),CISHIKENENGCHUXIANCHENDIAN,DANSHIBUYINGXIANGTIQUGUOCHENG,LIJICHUIDAHUNYUN,BUYAOLIXIN。

4. LIKEJIANGHUNHEWU(MEICIXIAOYU700μl,DUOKEYIFENLIANGCIJIARU)JIARUYIGEXIFUZHURAZHONG,(XIFUZHUFANGRUSHOUJIGUANZHONG)13,000 rpmLIXIN60MIAO,QIDIAOFEIYE。

5. JIA700μl QUDANBAIYERW1,SHIWENFANGZHI30MIAO,12,000rpm LIXIN30MIAO,QIDIAOFEIYE。

足球彩票RUGUODNACANLIUMINGXIAN,KEZAIJIARURW1HOUSHIWENFANGZHI5FENZHONGZAILIXIN。

足球彩票6. JIARU500μlPIAOXIYERW(QINGXIANJIANCHASHIFOUYIJIARUWUSHUIYICHUN!),12,000 rpm LIXIN30MIAO,QIDIAOFEIYE。JIARU500μlPIAOXIYERW,ZHONGFUYIBIAN。

足球彩票7. JIANGXIFUZHURAFANGHUIKONGSHOUJIGUANZHONG,13,000 rpmLIXIN2FENZHONG,JINLIANGCHUQUPIAOXIYE, YIMIANPIAOXIYEZHONGCANLIUYICHUNYIZHIXIAYOUFANYING。

8. QUCHUXIFUZHURA,FANGRUYIGERNase freeLIXINGUANZHONG,GENJUYUQIRNACHANLIANGZAIXIFUMODEZHONGJIANBUWEIJIA30-50μl RNase free water(SHIXIANZAI 70-90℃SHUIYUZHONGJIAREKETIGAOCHANLIANG), SHIWENFANGZHI1FENZHONG,12,000 rpm LIXIN1FENZHONG。

足球彩票9. RUGUOYUQIRNACHANLIANG>30μg,JIA30-50μl RNase free waterZHONGFUBUZHOU8, HEBINGLIANGCIXITUOYE,HUOZHESHIYONGCIDEXITUOYEJIAHUIDAOXIFUZHUZHONGFUBUZHOUYIBIAN(RUGUOXUYAORNANONGDUGAO)。

XITUOLIANGBIANDERNAXITUOYENONGDUGAO,FENLIANGCIXITUOHEBINGXITUOYEDERNACHANLIANGBIQIANZHEGAO15–30%,DANSHINONGDUYAODI,YONGHUGENJUXUYAOXUANZE。

    QQ在线客服
  •   在线咨询
电话
021-54845833
手机
021--54845833